BeaverBeads™ Mouse CD8+ Cell Isolation Kit (positive selection)
$0.00

Use BeaverBeads™ Mouse CD8+ Cell Isolation Kit (positive selection) to isolate CD8+ cells from mouse spleen cells of C57BL/6, label them with FITC anti-mouse CD8 antibody (clone number 53-5.8), and then analyze by flow cytometry.

  • Art.No.:70905-100
  • specification:for 1×10^9 cells
  • packaging:box

(I) Cell Isolation Kit Operation Flow Chart (Positive Selection)

Product Introduction

BeaverBeads™ mouse CD8+ Cell Isolation Kit (Positive selection) is intended for isolating CD8+ cells from mouse lymphoid organs. The principle involves labeling CD8+ cells with a CD8 capture antibody, followed by capturing the target cells using Releasable BeaverBeads™. The magnetic beads are then detached from the cell surface using BeaverBeads™ Release Buffer, resulting in magnetic bead-free CD8+ cells. The isolated CD8+ cells can be used for downstream molecular biology and cell biology experiments. The separation process requires the use of a magnetic separator.


Features

  • Purity reaches more than 95%.

  • Wide sample applicability, target cells can be captured directly.

  • Magnetic bead release technology, cells without magnetic beads labeling.


Application Cases

BeaverBeads™ Mouse CD4+ Cell Isolation Kit (positive selection)

Use the BeaverBeads Mouse CD4+ Cell Isolation Kit (positive selection) to isolate CD4+ cells from spleen cells of BALB/C mice, label them with PE anti-mouse CD4 antibody (clone number RM4-4), and then analyze by flow cytometry.

Fig.5 Flow cytometry analysis chart of mouse spleen cells isolation by BeaverBeads Mouse CD4+ Cell Isolation Kit (positive selection)


Conclusion: The purity of CD4+ cells before and after isolation was 13.3% and 97.2% respectively.


BeaverBeads™ Mouse CD8+ Cell Isolation Kit (positive selection)

Use BeaverBeads Mouse CD8+ Cell Isolation Kit (positive selection) to isolate CD8+ cells from mouse spleen cells of C57BL/6, label them with FITC anti-mouse CD8 antibody (clone number 53-5.8), and then analyze by flow cytometry.


Fig.6 Flow cytometry analysis chart of mouse spleen cells isolation by BeaverBeads Mouse CD8+ Cell Isolation Kit (positive selection)

Conclusion: The purity of CD8+ cells before and after isolation was 8.9% and 95.1% respectively.