▼ BeaverBeads™ for Nucleic Acid Extraction Kit
1.Can this product do select isolation?
Answer：Answer: This product cannot.
2.What's the proper size of DNA fragment for purification?
Answer：Answer: The kit is suitable to purify 100bp-50kb DNA.
3.What's the proper size of DNA fragment for recycle?
Answer：Answer: The kit is suitable to recycle DNA fragments over 100 bp and select from 100-3000bp DNA fragments.
4.If only PCR purification is performed, what's the adding ratio?
Answer：Answer: It is suggested to apply 1.8× to DNA purification.
5.Does the recommended screening rate in the instruction apply to the sample used？
Answer：Answer: No, the rate mentioned in the instruction is for samples dissolved in water, TE. If there are other salt cations (like Mg2+), PEG, glycerin and other substances in the sample, the size of selected DNA fragments will be affected.
6.How much can the kit extract per reaction?
Answer：Answer: The amounts of DNA in saliva samples from people can differ greatly. Usually 4-8 μg DNA can be extracted from 200 μL sample.
7.Is saliva DNA extraction kit only used for saliva samples？Is it also suitable for samples collected by swabs？
Answer：Answer: It is suitable to extract DNA from 0.2mL-1.0mL saliva samples or sampels collected by oral swab.
8.What are the suitable sample types？
Answer：Answer: It is suitable to extract viral DNA/RNA from plasma, serum, urine, secretion, cell culture supernatant, virus preservation fluid and saliva samples.
9.Can this kit match automated nucleic acid extraction instrument？
Answer：Answer: it can match the majority of magnetic bar nucleic acid extraction device or pipette workstation in the market.
10.How much can the kit extract per reaction?
Answer：Answer: 3-7μg mini plasmid can be acquired each reaction.
11.Is it able to do extraction of large volume of samples?
Answer：Answer: No, the kit is only suitable for the extraction of 1-4mL sample.
12.What's the difference between 70406 and 70406Ⅱ?
Answer：Answer: 70406 is for manual extraction; 70406Ⅱ is for automate extraction, like Rosseta 24 and KingFisher Flex 24.
13.What's the appropriate amount of serum/blood?
Answer：Answer: It is suitable to extract cfDNA from 0.2mL-5mL serum/blood sample.
14.What's the recycling efficiency of cell free DNA?
Answer：Answer: Recycling efficiency can be higher than 85%.
15.Whether the kit can perform DNA select isolation？
Answer：Answer: This kit cannot. To avoid the effects of genome fragments，Beaver Cell Free DNA Tubes are recommended (Cat.No. 43803).
16.What's the extraction efficiency？
Answer：Answer: The amount of extracted cfDNA from serum samples of normal people is about 10 ng/mL (The amount of cfDNA in normal human plasma is 10-25 ng/mL).
17.Is it able to perform automate operations?
Answer：Answer: The automatic pipette type nucleic acid extraction device has been tested. The kit is suitable for Rosseta 32 and etc..
▼ BeaverBeads™ for Protein Purification
▼ BeaverBeads™ for Immunoprecipitation
1.How about the homogeneity of 1μm SA bead？Test method.
Answer：Answer: CV<5%，scanning electron microscopy.
2.How to separate SA magnetic beads from biotin-labeled biological ligands？
Answer：Answer: Scheme 1: 0.1% SDS，boil for 5min; Scheme 2： pH=8.2, in 10mM EDTA containing 95% formamide，heat under 65℃ 5min or 90℃ 2min. Expulsion rate will be 95%.
3.How to choose between Mag NHS and Magrose NHS？
Answer：Answer: Mag NHS beads have higher sensitivity which is used for test and immunoprecipitation. Magrose NHS beads have high capacity which is used for purification.
4.What are the experimental and judgemental methods of determining the appropriate Coupling Buffer?
Answer：Answer: To facilitate the operation of the experiment, the original protein preservation solution without small amino molecules can be applied. PBS or MES is commonly utilized.
▼ BeaverBeads™ SiO2/Polymer/Magrose
1.What should I pay attention to for ligand coupling？
Answer：Answer: a. It is necessary to ensure that the ligand preservation system contains no other primary amino components（like Tris, BSA, gelatin, etc.). If it does, dialysis or ultrafiltration is required. b. A protein solution of 0.1-3.0 mg/mL is generally used in the coupling of proteins. The higher the concentration is, the higher the efficiency of the couple will be.
2.What is the amount of 1μm beads per mg?
Answer：Answer: About 10^8
3.What is the amount of antibody can 1μm bead couple with (70104)？
Answer：Answer: About 25-30μg/mg.
4.For DNA extraction, which magnetic beads are more recommended?
Answer：Answer: For gene extraction, plasmid, etc.，Mag OH-500 is more recommended (70301 or 70304). For small pieces of nucleic acid (Viral DNA/RNA, Circulating DNA, etc.), COOH beads are more recommended (70107 or 70108).
5.What fields can Magrose OH be applied in？
Answer：Answer: Clients can perform the surface modification of Magrose OH and then apply it in protein purification.
6.How much nucleic acid can the beads bind？
Answer：Answer: More than 20μg DNA/mg beads.
▼ Sample Collection and Storage
▼ TC-Treated Cell Culture Series
1.What is the concentration range of detectable biotin-labeled IgG？
2.What is the maximum binding amount of Biotin-labeled IgG？
Answer：Answer: About 70 ng/well
3.What are the suitable cell types of Suspension Cell Culture Surface？
Answer：Answer: ①Suspension culture of adherent and semi-adherent cells ②Sphere cultivation of tumor cells
4.Compared with other brands，are there any advantages of Beaver TCT Cell Culture Series？
Answer：Answer: Beaver TCT Series have higher cell adherence rate and cell viability, so that are more suitable for adherent cell growth.