1. High Production: The optimized operation procedure to maximize the extraction of circulating DNA;
2. High purity: The optimized buffer system to remove PCR inhibitors and Protein contaminants effectively;
3. Minimum elution volume: Elution volume is about 20μL to ensure higher nucleic acid concentration;
4. Flexible sample volume: extract circulating DNA from 0.2~5 ml serum or plasma.
Recovery rate of DNA fragments from different sample volumes
Add 151bp Nucleic acid fragment into blood to make concentration of 10ng/μL, then use Beaver Circulating Extraction Kit to recover nucleic acid fragment from different sample volumes and quantify it with qPCR. According to research data, recovery rate of Beaver Product can exceed 85% in any 0.2~5 mL samples.
qPCR amplification curves from the same sample
Add 151bp Nucleic acid fragment into blood to make concentration of 10ng/mL, then use Beaver Circulating Extraction Kit recover nucleic acid fragment from the sample and quantify it with qPCR. According to research data, Ct values are consistent, which shows this kit has good stability.
Circulating DNA obtained from three peripheral blood plasma samples. Then use Qubit to find the concentration. The result shows that Beaver product has a better performance than the competitor.