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Product Introduction
BeaverBeads™ mouse CD8+ T cell isolation kit is intended for negative isolation of CD8+ T cells from mouse lymphoid organs. The isolated CD8+ T cells are highly pure and viable cells. The principle is to add biotin labeled monoclonal antibodies to your cell suspension to label non-target cells (non-CD8+ T cells), which can be captured by the streptavidin labeled magnetic beads. The magnetic labeled fraction is retained when using a magnetic separator, while untouched bead- and antibody-free CD8+ T cells can be easily collected from the supernatant.
Product Information
Application Cases
Use BeaverBeadsTM Mouse CD8+ T Cell Isolation Kit to isolate CD8+ T cells from mouse spleen cells, label them with FITC anti-mouse CD8 antibody (clone number 53-6.7), and then analyze by flow cytometry.
Fig.3 Flow cytometry analysis chart of mouse spleen cells isolation by BeaverBeadsTM Mouse CD8+ Cell Isolation Kit (negative selection)
Conclusion: The purity of CD8+ T cells before and after isolation was 13.8% and 97.1% respectively.
