The principle of magnetic beads cell isolation is to use immunomagnetic beads to label cells, the cells bound to the magnetic beads and without bound to the magnetic beads are separated by an external magnetic field. BeaverBeads Cell Isolation Kit has two separation technologies, negative selection and positive selection, for both human and mouse samples, and is suitable for different application directions.
- Art.No.:70904-10
- specification:for 1 x 10^8 cells
- packaging:box
Product Introduction
BeaverBeads™ mouse CD4+ Cell Isolation Kit (Positive selection) is intended for isolating CD4+ cells from mouse lymphoid organs. The principle involves labeling CD4+ cells with a CD4 capture antibody, followed by capturing the target cells using Releasable BeaverBeads™. The magnetic beads are then detached from the cell surface using BeaverBeads™ Release Buffer, resulting in magnetic bead-free CD4+ cells. The isolated CD4+ cells can be used for downstream molecular biology and cell biology experiments. The separation process requires the use of a magnetic separator.
Product Information
Application Cases
Use the BeaverBeads™ Mouse CD4+ Cell Isolation Kit (positive selection) to isolate CD4+ cells from spleen cells of BALB/C mice, label them with PE anti-mouse CD4 antibody (clone number RM4-4), and then analyze by flow cytometry.
Fig.2 Flow cytometry analysis chart of mouse spleen cells isolation by BeaverBeadsTM Mouse CD4+ Cell Isolation Kit (positive selection)
Conclusion: The purity of CD4+ cells before and after isolation was 13.3% and 97.2% respectively.
