The Streptavidin Coated Beads, based on superparamagnetic or non-magnetic beads covalently coated with streptavidin, are designed to bind a variety of biotinylated molecules in immunoprecipitation, immunoassay(ELISA), cell sorting, protein interaction studies, protein/DNA hybridization reaction or even DNA sequencing application.
- Art.No.:22309-10
- specification:10 mg/mL, 2.8 μm
- packaging:10 mL, 10 mg/mL, 2.8 μm
BeaverBeadsTM Streptavidin 280
1. Introduction
(SA-Biotin) system has extremely high binding affinity (Kd=10^-15), It has a wide range of applications in the biological field. BeaverBeadsTM Streptavidin covalently connects the SA to the solid carrier surface by using the patented protein coupling technique of Beaver, which can efficiently bind the ligands such as biotinylated antibodies, nucleic acids, proteins etc. The product adopts superparamagnetic microspheres with uniform size and regular morphology, which facilitates the rapid and convenient capture of target molecules and the realization of magnetic separation. This product can be equipped with automation equipment for high flux operation.
2. Basic Information
Index | Specification |
Bead Size | 2.8 μm |
Color | Brown |
Suspension time | >30 min |
Biotinylated single stranded oligonucleotide (24nt ) | 600 pmol/mg beads |
Biotinylated IgG | 20 μg/mg beads |
3. Figure
4. Acridine ester direct chemiluminescence
Compare | Beaver | |
S0 | 1045401 | 971434 |
S1 | 555277 | 538365 |
S2 | 360392 | 365044 |
S3 | 225030 | 227096 |
S4 | 135909 | 140109 |
S5 | 73068 | 81898 |
Clinic 1 | 374920 | 375358 |
Clinic 2 | 151969 | 160816 |
On FT4, BeaverBeadsTM shares the close measured value with that of the control magnetic beads, and the ratio is good。
5. ALP indirect chemiluminescence
Case 1 DAS-ELISA（HRP）MYO Test
MYO Standard ng/mL | BEAVER | Competitor | |
250.0 | 64903596 | 50156262 | |
125.0 | 41826343 | 33255404 | |
62.5 | 22886909 | 18280646 | |
31.2 | 11346377 | 8803140 | |
15.6 | 5183601 | 3937511 | |
7.8 | 2417043 | 1771504 | |
3.9 | 1070908 | 770467 | |
0 | 6947 | 7017 | |
BeaverBeadsTM chemiluminescence performance is comparable to that of competitors’ magnetic products, which can be replaced, especially for low value samples, with better separation and more sensitive detection.
Case 2 DAS-ELISA（AP）tlgE Test
Conclusion：After calibration，the overall signal of BeaverBeadsTM is consistent with that of competing products, and linear regression R and slope are also ideal.
6. Electrochemistry（PCT，DAS-ELISA）
PCT（DAS-ELISA） | ||
Concentration（ng/mL） | Compare | Beaver SA |
0.026 | 956.7 | 1212 |
0.04 | 1038 | 1614 |
0.095 | 1379 | 3676 |
0.434 | 3841 | 15379 |
0.829 | 7048 | 32097 |
4.26 | 37719 | 183974 |
8.71 | 87416 | 326360 |
16.78 | 180826 | 666253 |
34.76 | 404879 | 1377481 |
62.78 | 775087 | 2375171 |
100 | 1313548 | 3969934 |
Conclusion：In PCT, BeaverBeadsTM has higher chemiluminescence signal, better separation and more sensitive detection.
7. Product List
Product Name | Size | Cat. No. |
BeaverBeadsTM COOH 280 | 2.8μm | 70109 |
BeaverBeadsTM Streptavidin 280 | 2.8μm | 22309 |
BeaverBeadsTM Streptavidin | 300 nm | 22308 |
BeaverBeadsTM Streptavidin | 1μm | 22307 |
