The principle of magnetic beads cell isolation is to use immunomagnetic beads to label cells, the cells bound to the magnetic beads and without bound to the magnetic beads are separated by an external magnetic field.. BeaverBeads Cell Isolation Kit has two separation technologies, negative selection and positive selection, for both human and mouse samples, and is suitable for different application directions.
(I) Cell Isolation Kit Operation Flow Chart (Negative Selection)
Product Introduction
The negative selection kit uses nano magnetic beads to label and remove non-target cells, and then obtaining target cells that are not labeled by magnetic beads and antibodies.
Features
l Simple and fast: The target cells can be isolated in 15 mins.
l No separation column required: The target cells can be quickly separated using a magnetic separator.
l High purity: The purity of isolated cells can reach more than 95%.
l High activity: after isolation, more than 99% of viable cells can be obtained, and the cells have good function.
Application Cases
BeaverBeads Human CD3+ T Cell Isolation Kit (negative selection)
Use BeaverBeads human CD3+ T Cell Isolation Kit to isolate CD3+ T cells from human PBMC, label them with PE anti-human CD3 antibody (clone number OKT3) and then analyze by flow cytometry.
Fig.1 Flow cytometry analysis chart of human PBMC cells isolation by BeaverBeadsTM Human CD3+ Cell Isoaltion Kit (negative selection)
Conclusion: The purity of CD3+ T cells before and after isolation was 69.1% and 97.9% respectively.
BeaverBeads Mouse CD3+ T Cell Isolation Kit (negative selection)
Use BeaverBeads Mouse CD3+ T Cell Isolation Kit to isolate CD3+ T cells from mouse spleen cells, label them with FITC anti-mouse CD3 antibody (clone number 145-2C11), and then analyze by flow cytometry.
Fig.2 Flow cytometry analysis chart of mouse spleen cells isolation by BeaverBeads Mouse CD3+ Cell Isolation Kit (negative selection)
Conclusion: The purity of CD3+ T cells before and after isolation was 35.3% and 97.6% respectively.
BeaverBeads Mouse CD4+ T Cell Isolation Kit (negative selection)
Use BeaverBeads Mouse CD4+ T Cell Isolation Kit to isolate CD4+ T cells from mouse spleen cells, label them with FITC anti-mouse CD4 antibody (clone number GK1.5) and then analyze by flow cytometry.
Fig.3 Flow cytometry analysis of mouse spleen cells isolation by BeaverBeads Mouse CD4+ Cell Isolation Kit (negative selection)
Conclusion: The purity of CD4+ T cells before and after isolation was 20.3% and 98.8% respectively.
BeaverBeads Mouse CD8+ T Cell Isolation Kit (negative selection)
Use BeaverBeads Mouse CD8+ T Cell Isolation Kit to isolate CD8+ T cells from mouse spleen cells, label them with FITC anti-mouse CD8 antibody (clone number 53-6.7), and then analyze by flow cytometry.
Fig.4 Flow cytometry analysis chart of mouse spleen cells isolation by BeaverBeads Mouse CD8+ Cell Isolation Kit (negative selection)
Conclusion: The purity of CD8+ T cells before and after isolation was 13.8% and 97.1% respectively.
