1. Automation solution for 200μL-10 ml samples;
2. High extraction: > 2μg from 200mg samples;
3. Excellent stability;
4. No toxic solvent: such as no phenol and chloroform.
1. Recovery rate
Fig. 1 Recovery rate of DNA fragments from different samples
Add exogenous DNA fragment to stool samples, then use Beaver Stool DNA Kit to recover nucleic acid fragment and quantify it with qPCR.(see Fig. 1). The results showed that the recovery rate of the target fragment with Beaver products was between 40% and 70% in different samples.
2. Stability test
Fig. 2: qPCR amplification curves from the same sample
Add exogenous DNA fragment to stool samples, then use Beaver Stool DNA Kit to recover nucleic acid fragment several times from the same sample and quantify it with qPCR.(see Fig. 2). According to research data, Ct values are consistent, which shows this kit has good stability.
3. Extraction Rate
Fig. 3 Total DNA extracted by Beaver product from different stool samples
Using Beaver Stool DNA Kit to extract DNA from different human stool samples and use Qubit equipment to test DNA content(see Fig. 3). The result showed Beaver DNA yield >2μg.
4. Average Comparison
Fig. 4 Average sample extraction from different brands
Use different brand stool DNA extraction kit to extract the same 200 mg sample, average extracted amounts are showed in Fig. 4. Beaver Stool DNA extraction kit performance is superior to others’. Beaver Stool DNA Extraction Kit has excellent extraction efficiency.