BeaverBeads™ Streptavidin
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The Streptavidin Coated Beads, based on superparamagnetic or non-magnetic beads covalently coated with streptavidin, are designed to bind a variety of biotinylated molecules in immunoprecipitation, immunoassay(ELISA), cell sorting, protein interaction studies, protein/DNA hybridization reaction or even DNA sequencing application.

  • Art.No.:22309-10
  • specification:10 mg/mL, 2.8 μm
  • packaging:10 mL, 10 mg/mL, 2.8 μm

BeaverBeadsTM Streptavidin 280

 

1. Introduction

(SA-Biotin) system has extremely high binding affinity (Kd=10^-15), It has a wide range of applications in the biological field. BeaverBeadsTM Streptavidin covalently connects the SA to the solid carrier surface by using the patented protein coupling technique of Beaver, which can efficiently bind the ligands such as biotinylated antibodies, nucleic acids, proteins etc. The product adopts superparamagnetic microspheres with uniform size and regular morphology, which facilitates the rapid and convenient capture of target molecules and the realization of magnetic separation. This product can be equipped with automation equipment for high flux operation.

2. Basic Information

Index

Specification

Bead Size

2.8 μm

Color

Brown

Suspension time

>30 min

Biotinylated single stranded oligonucleotide (24nt )

600 pmol/mg beads

Biotinylated IgG

20 μg/mg beads

3. Figure

 

 

4. Acridine ester direct chemiluminescence



Compare

Beaver

S0

1045401

971434

S1

555277

538365

S2

360392

365044

S3

225030

227096

S4

135909

140109

S5

73068

81898

Clinic 1

374920

375358

Clinic 2

151969

160816


On FT4BeaverBeadsTM shares the close measured value with that of the control magnetic beads, and the ratio is good。

5. ALP indirect chemiluminescence

Case 1 DAS-ELISA(HRPMYO Test

MYO Standard

ng/mL

BEAVER

Competitor

250.0

64903596

50156262

125.0

41826343

33255404

62.5

22886909

18280646

31.2

11346377

8803140

15.6

5183601

3937511

7.8

2417043

1771504

3.9

1070908

770467

0

6947

7017

 

BeaverBeadsTM chemiluminescence performance is comparable to that of competitors magnetic products, which can be replaced, especially for low value samples, with better separation and more sensitive detection.


Case 2 DAS-ELISA(AP)tlgE Test

 

ConclusionAfter calibrationthe overall signal of BeaverBeadsTM is consistent with that of competing products, and linear regression R and slope are also ideal.

6.  Electrochemistry(PCT,DAS-ELISA)

PCT(DAS-ELISA)

Concentration(ng/mL)

Compare

Beaver SA

0.026

956.7

1212

0.04

1038

1614

0.095

1379

3676

0.434

3841

15379

0.829

7048

32097

4.26

37719

183974

8.71

87416

326360

16.78

180826

666253

34.76

404879

1377481

62.78

775087

2375171

100

1313548

3969934

 

 

 

ConclusionIn PCT, BeaverBeadsTM has higher chemiluminescence signal, better separation and more sensitive detection.

7. Product List

Product Name

Size

Cat. No.

BeaverBeadsTM COOH 280

2.8μm

70109

BeaverBeadsTM Streptavidin 280

2.8μm

22309

BeaverBeadsTM Streptavidin

300 nm

22308

BeaverBeadsTM Streptavidin

1μm

22307