BeaverBeads™ Mag NHS
Art.No. Specification List Price Qty Action
70701-50 50 mL, 10 mg/mL, 2 μm Inquiry

Product Introduction

The NHS magnetic beads are superparamagnetic beads with activated groups of N_Hydroxysuccinimide on the surface, which can react with primary amines on desired ligands and bind them covalently with amide linkage to the beads. The ligand can be various molecules including antigen, antibody and other proteins. Compared with the traditional carboxyl, amino magnetic beads, the NHS group of magnetic beads don’t need to activate EDC/NHS or Glutaraldehyde, simply dissolving the amino-containing bio-ligand in the coupling buffer, at room temperature, mixing with proteins and the NHS magnetic beads for only 1-2 hours, the bio-ligand can be coupled to the magnetic beads. The advantages include easy operation, mild coupling conditions and coupling fast and efficient. The magnetic bead coupling process must be carried out in a buffer solvent free of any amino. This method can be operated manually or automatically.

 

Product Information

1.       Product basic information

Product   information

BeaverBeadsTM   Mag NHS

Beads size

-2 µm (Monodisperse)

Binding   Capacity

≥ 30 µg rabbit IgG/mg beads

Concentration

10 mg/mL

Preservative

DMAC

Shelf   Life

24 months at 2-8 ºc

Note: The binding ability is related to the characteristics of the bio-ligand itself, values here are only for reference

 

2.       BeaverBeadsTM Mag NHS binding capacity with other proteins

Protein

Molecular   Weight(kDa)

NHS beads   binding capacity (ug/mg of beads)

IgG

150

50

Streptavidin

53

24

Protein A/G

50

21

Protein L

36

39

Note: The binding amount will be influenced by the number of protein Active amine Group and secondary Amine group.