BeaverBeads® Human CD8⁺ Cell Isolation Kit (PositiveSelection)
The Human CD8⁺ Cell Isolation Kit (PositiveSelection) is intended for the isolation of CD8⁺ cells from human peripheral blood mononuclear cells (PBMCs). The purified CD8⁺ cells can be applied in downstream molecular and cellularbiology experiments. Target cells are labelled with CD8 Capture Antibody, captured by BeaverBeads®, and the magnetic beads are subsequently dissociated from the cell surface using Release Buffer to obtain beadfree human CD8⁺ cells.
Product Features
Streamlined Operation: No separation column is required; cell isolation can be completed with a magnetic separator.
High Purity: Delivers high separation purity, with postisolation CD8⁺ cell purity up to 97%.
High Viability: The isolation procedure maintained cell viability, yielding bead-free CD8⁺ cells that retained their biological functions.
Performance Data
1. Cell Purity Analysis
CD8⁺ cells were isolated from human peripheralblood PBMCs using the BeaverBeads® Human CD8⁺ Cell Isolation Kit (PositiveSelection). Cells before and after isolation were stained with PEconjugated antihuman CD8a antibody (Clone: RPAT8) and APCconjugated antihuman CD3 antibody (Clone: OKT3), followed by flow cytometric analysis. As shown in Figure 1, CD8⁺ cells (CD3⁺CD8⁺) accounted for 12.8 % in the initial PBMC sample, and the purity of isolated CD8⁺ cells reached 95.4 % (95.8±1.1 %).
Figure 1. Flowcytometric analysis of CD8⁺cell
2. Cell Viability Analysis
CD8⁺ cells were isolated from human peripheralblood PBMCs using the BeaverBeads® Human CD8⁺ Cell Isolation Kit (PositiveSelection). Cells pre and postisolation were stained with 7AADPerCP, and cell viability was analyzed by flow cytometry. As shown in Figure 2, the viability of starting cells was 98.0 %, while postisolation cell viability was 97.4 %.
Figure 2. Flowcytometric analysis of CD8⁺cell viability
3. CellActivation Validation
CD8⁺ cells were isolated from human peripheralblood PBMCs with the BeaverBeads® Human CD8⁺ Cell Isolation Kit (PositiveSelection). Purified cells were cocultured with BeaverBeads® Human CD3/CD28 TCell Activation Beads (Cat. No. 70910). After 24 hours, the proportion of CD25⁺CD69⁺ Tcells was measured by flow cytometry.As shown in Figure 3, the percentage of CD25⁺CD69⁺ Tcells among kitisolated CD8⁺ cells was 0.01 % in the unstimulated group and increased to 57.3 % after stimulation.
Figure 3. Flowcytometric analysis of surface CD25 and CD69 expression on CD8⁺ cells
