BeaverBeads® Mouse NK Cell Isolation Kit
This product is designed for the isolation of natural killer (NK) cells from mouse spleen samples. It adopts a negativeselection workflow: magnetic beads and monoclonal antibodies are used to label and deplete nontarget cells (nonNK cells), so as to isolate mouse NK cells. Since NKcell surface antigens are not directly labelled during separation, the activation status and biological functions of NK cells are preserved to the maximum extent. The isolated NK cells can be used in downstream molecular and cellular biology experiments.
Product Features
High Purity: The purity of isolated mouse NK cells can reach up to 91%.
High Viability: Obtain antibodyfree mouse NK cells with wellpreserved biological functions.
Easy Operation: No separation column is required; cell isolation can be completed simply with a magnetic separator.
Performance Data
1. Purity Detection of Isolated NK Cells
NK cells were isolated from splenocytes of C57BL/6 mice using the BeaverBeads® Mouse NK Cell Isolation Kit. Cells before and after isolation were stained with PE anti‑mouse CD3 (Clone: 500A2) and FITC anti‑mouse CD49b (Clone: DX5), followed by flow cytometric analysis. As shown in Figure 1, NK cells (CD3⁻CD49b⁺) account for approximately 4% in the initial spleen sample, while the purity of isolated NK cells reaches 91.57% (91.53±1.78%).
Figure 1. Flow cytometric analysis of NKcell purity pre and postisolation
2. Cell Viability Analysis After Isolation
(1) NK cells were isolated from splenocytes of C57BL/6 mice using the BeaverBeads® Mouse NK Cell Isolation Kit. Cells before and after isolation were stained with 7‑AAD and subjected to flow cytometry for viability assessment. As shown in Figure 2, the viability of starting splenocytes was 97.98%, whereas post‑isolation cell viability was 91.90%.
Figure 2. Flow cytometric detection of cell viability pre‑ and post‑isolation
(2) NK cells were isolated from splenocytes of C57BL/6 mice using the BeaverBeads® Mouse NK Cell Isolation Kit. Viability of the sorted CD49b⁺ cell population was further analysed. Cells before and after isolation were stained with 7‑AAD for flow‑cytometry viability measurement. As shown in Figure 3, within the CD49b⁺ gated population, the pre‑isolation cell viability was 82.54%, which increased to 95.38% after negative selection.
Figure 3. Flow cytometric viability analysis of CD49b⁺ cells pre‑ and post‑isolation)
3. Functional Assay of Isolated NK Cells
NK cells were isolated from splenocytes of C57BL/6 mice using the BeaverBeads® Mouse NK Cell Isolation Kit. Purified NK cells were stimulated with Cell Stimulation Cocktail (containing PMA, Ionomycin, Brefeldin A and Monensin) for 4‑6 h. Afterwards, surface CD107a (LAMP‑1) and intracellular IFN‑γ expression were determined by flow cytometry. As shown in Figure 4, upon stimulation with Cell Stimulation Cocktail, NK cells exhibited markedly up‑regulated CD107a expression and elevated IFN‑γ secretion, indicating enhanced degranulation activity and cytokine‑secreting capacity.
Figure 4. Upregulated CD107a and IFNγ expression in NK cells after Cell Stimulation Cocktail treatment
4. Performance Comparison with Competitor Kits
NK‑cell purity, viability and yield obtained with the BeaverBeads® Mouse NK Cell Isolation Kit were compared with those of commercial competitor NK‑cell isolation kits.
(1) NK cells were isolated from splenocytes of C57BL/6 mice. Cells before and after isolation were stained with PE anti‑mouse CD3 (Clone: 500A2) and FITC anti‑mouse CD49b (Clone: DX5) for flow cytometric analysis. As shown in Figure 5, the NK‑cell purity achieved by the BEAVER kit exceeded that of Competitor I and Competitor II.
Figure 5. Comparison of NK‑cell negative‑selection purity between the BEAVER kit and competitor kits
(2) NK cells were isolated from C57BL/6 mouse splenocytes using the BeaverBeads® Mouse NK Cell Isolation kit and competitor kits respectively, and post‑isolation cell viability was compared. As shown in Figure 6, the cell viability of NK cells isolated by the BEAVER kit was superior to both Competitor I and Competitor II.
Figure 6. Comparison of NK‑cell viability after isolation using the Beaver kit and competitor kits
(3) NK cells were isolated from C57BL/6 mouse splenocytes using the BEAVER kit and competitor kits respectively, and cell yield was compared. As shown in Figure 7, the NKcell yield of the BEAVER kit (67.33±8.19%) outperformed Competitor I and Competitor II.
Figure 7. Comparison of NKcell negativeselection yield between the Beaver kit and competitor kits
