CD4+ T cells were isolated from human peripheral blood mononuclear cells (PBMCs) using the BeaverBeads™ Human CD4+ T Cell Isolation Kit (negative selection).
- Art.No.:70908-50
- specification:for 5×10^8 cells
- packaging:box
Product Introduction
BeaverBeads™ human CD4+ T cell isolation kit is intended for negative isolation of CD4+ T cells from human peripheral blood mononuclear cells (PBMCs). The isolated CD4+ T cells are highly pure and viable cells. The principle is to add biotin labeled monoclonal antibodies to your cell suspension to label non-target cells (non-CD4+ T cells), which can be captured by the streptavidin labeled magnetic beads. The magnetic labeled fraction is retained when using a magnetic separator, while untouched bead-and antibody-free CD4+ T cells can be easily collected from the supernatant.
Product Information
Example of a separation
CD4+ T cells were isolated from a PBMC suspension using BeaverBeadsTM human CD4+ T cell isolation kit. The cells before and after isolation were stained with PE anti-human CD4 antibody (clone RPA-T4) and APC anti-human CD3 (clone OKT3) and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and 7-AAD fluorescence.
