(I) Cell Isolation Kit Operation Flow Chart (Positive Selection)
Product Introduction
The positive selection kit uses nano magnetic beads to label and enrich target cells, and then uses a release agent to dissociate the magnetic beads from the cell surface to obtain target cells without magnetic bead labeling.
Features
l Purity reaches more than 95%.
l Wide sample applicability, target cells can be captured directly.
l Magnetic bead release technology, cells without magnetic beads labeling.
Application Cases
BeaverBeads™ Mouse CD4+ Cell Isolation Kit (positive selection)
Use the BeaverBeads Mouse CD4+ Cell Isolation Kit (positive selection) to isolate CD4+ cells from spleen cells of BALB/C mice, label them with PE anti-mouse CD4 antibody (clone number RM4-4), and then analyze by flow cytometry.
Fig.5 Flow cytometry analysis chart of mouse spleen cells isolation by BeaverBeads Mouse CD4+ Cell Isolation Kit (positive selection)
Conclusion: The purity of CD4+ cells before and after isolation was 13.3% and 97.2% respectively.
BeaverBeads™ Mouse CD8+ Cell Isolation Kit (positive selection)
Use BeaverBeads Mouse CD8+ Cell Isolation Kit (positive selection) to isolate CD8+ cells from mouse spleen cells of C57BL/6, label them with FITC anti-mouse CD8 antibody (clone number 53-5.8), and then analyze by flow cytometry.
Fig.6 Flow cytometry analysis chart of mouse spleen cells isolation by BeaverBeads Mouse CD8+ Cell Isolation Kit (positive selection)
Conclusion: The purity of CD8+ cells before and after isolation was 8.9% and 95.1% respectively.
